Bee beautiful

Our conservator Bethany Palumbo tells us how she restored a beautiful 19th-century papier-mâché model of a honeybee hive, created by master model-maker and anatomist Louis Thomas Jérôme Auzoux

Louis Thomas Jérôme Auzoux

Although the Museum’s collections are mostly of organic specimens, we also hold a fascinating collection of scientific models made to represent the natural world, made from all types of materials, from wax and cardboard to plaster and paint.

We are lucky enough to own a model made by esteemed French anatomist Louis Auzoux (1797-1880), who in the late 19th century developed a method of building strong yet light papier-mâché models that could be taken apart and rebuilt, allowing internal elements such as tissues and organs to be studied in detail.

Model of a honeybee hive in box with six bees, by Louis Auzoux

While Auzoux made many models demonstrating human anatomy, he later expanded his business to include magnified models of plants and insects. The model we have is of a honeybee hive, containing six beautiful bees.

The hive, painted with a protein-based paint and varnished with gelatine, is large enough to allow the viewer to see the fine details of the hive, including individual chambers containing tiny larvae.

As you can see in the image at the top of the article, the bees themselves are also intricately painted, with rabbit hair used to simulate their natural fuzz, and delicate wings constructed from metal wire.

While there was much to admire about this model, it was in received in poor condition. Previous restoration attempts had introduced many materials that were now failing. There were fills, constructed of paper, applied to areas in an attempt to hide cracks in the original model. These were covered in oil paint, which was dripping over the original paintwork and had become brittle and discoloured.

Oil paint layers were peeling from the model

The whole hive was coated in a layer of cellulose nitrate film, a popular coating in the mid-20th century which was used as protection and to create a gloss finish. This coating doesn’t age well, resulting in peeling. It had also been applied to the bees themselves, clumping together the bee ‘fuzz’ and disguising the paintwork underneath.

The priority for treatment was to return the model to its original form while stabilising it for the future.

I undertook treatment in several stages over the course of six months. First, the cellulose nitrate film was removed from all areas using acetone, which could be applied with a cotton bud and fortunately didn’t affect the paint layer beneath.

Fill material used to cover previous damage had become discoloured

The next stage was to remove the discoloured oil paint from the hive. This was done manually using metal and wooden tools lubricated with white spirit, which were used to gently scrape the surface under magnification. This revealed old fills on the hive, made from a combination of plastic tape, paper and old adhesives which also needed to be removed. They were easily softened with water and gently peeled away.

Once all unstable introduced materials were removed, work began to stabilise the original model. The bees were suffering from paint cracking and peeling, as seen in the magnified photograph below.

Peeling paint at 6x magnification

We decided to consolidate this using gelatine as it would be in keeping with the original construction and could easily be reversed if necessary. Gelatine was mixed in water and warmed to make it a thin consistency, and then applied with a paintbrush. Once the paint flakes had softened they could be gently pressed down. Gelatine was also used with acid-free tissue to stabilise the cracks and areas of surface loss on the hive.

With the hive and bees now clean and stable, the quality of this piece and its incredible paintwork can really be admired. We hope to put it on display soon for all our visitors to enjoy!

Image credit - Gilles San Martin, licensed under CC BY-SA 2.0

Decoding the honeybee dance

Image credit - Gilles San Martin, licensed under CC BY-SA 2.0

This article is taken from European research magazine Horizon as part of our partnership to share natural environment science stories with readers of More than a Dodo.

Unravelling one of the most elaborate forms of non-human communication – the honeybee’s waggle dance – could help researchers better understand insect brains and make farming more environmentally friendly.

It’s part of a field of work looking at insect neurology which is helping to unravel the complexity of their brains.

Bees have evolved a unique, and ingenious, way to communicate with each other – the waggle dance. By shaking their abdomens in a particular way, a bee can tell others in its hive the specific direction and distance of a food source or a new site for a nest.

‘If nectar or pollen is in the direction of the sun, a bee will run a figure of eight that is orientated towards the top of the hive. If pollen is found 90 degrees from the sun they will point that way instead,’ explained Dr Elli Leadbeater, a bee expert from the School of Biological Sciences at the University of London, in the UK.

The longer the bees spend dancing corresponds to the better quality of a food source, while the more time spent on each figure eight represents the distance from the pollen or nectar.

Researchers now believe that decoding this information-packed dance further could reveal a link between bees’ brains and how the surrounding environment affects them. In a project called BeeDanceGap, Dr Leadbeater is working to identify the exact genes in the bee brain that play a role in helping the insects understand this waggle dance.

To do this, researchers must first identify the best dancing bees in a test hive and watch them as they reveal a food source to other worker bees. The newly educated bees are then captured as they leave the hive so their brain tissue can be genetically analysed to determine which genes associated with learning and memory were activated from following the waggle dance.

Only a few individuals are used in this way and the genetic data provides a deep insight into the neurology of a bee’s brain – at a time crucial to their future.

The observation bee hive at the Oxford University Museum of Natural History gives visitors a glimpse into hive life.

Collapse

Beekeepers around the world have reported that many of their bees leave and never come back, causing hives to suddenly collapse. Experts believe there are several factors contributing to this widespread loss of bee colonies, including climate change, parasites and habitat loss. Agrichemicals like pesticides and neonicotinoids, which are used to kill unwanted insects on farms, have also been strongly linked to the problem.

‘The rate pesticides or neonicotinoids are applied to crops don’t necessarily kill bees but they make them worse at foraging,’ said Dr Leadbeater.

If you do damage to just one part of the brain of a lot of individual bees, it can have huge consequences for the whole colony.
Dr Elli Leadbeater, University of London, UK

Neonicotinoid pesticides have been found to bind to parts in the insect brain, disrupting neural transmission. This leads to some brain cells either failing to develop or not functioning properly.

The EU recently banned neonicotinoids, which Dr Leadbeater believes is a huge step forward in protecting bees, but she said governments still need more rigorous ‘long-term environmental safety monitoring’. Without this, there is a risk that other agricultural products used in place of neonicotinoids could impact honeybees in a similar way.

But when the first results of BeeDanceGap are published later this year, they could contribute to building better criteria for testing future agriculture practices or products. Dr Leadbeater believes it will provide a new understanding of a bee’s brain, and so help identify problems sooner.

The impacts of quickly identifying problems go far further than just supporting beekeepers and their insect charges. Protecting honeybees, along with bumblebees and wild bees, is also essential to maintain a healthy and productive environment. These insects pollinate over 80% of crops and wild plants in Europe. According to Professor Martin Giurfa, from the Research Center on Animal Cognition at CNRS in France, ‘preserving little brains is about preserving biodiversity’.

Honey bees working inside a hive

More than machines

Honeybees have a higher social complexity than many other species. Alongside the waggle dance communication, each hive has a division of labour where different workers have responsibility for a variety of tasks – such as foraging for pollen, nursing the young, building hives and even removing the dead.

Prof. Giurfa is co-leading the BrainiAnt project, which looks at how this type of complex social behaviour evolved and how it affected the structure of insect brains. He said that when ‘you understand how bees perceive the world, it is easier to find ways to protect them’.

Through the work of researcher Dr Sara Arganda, the project is investigating a part of the insect brain called the mushroom body, where learning occurs and long-term memories are stored. Researchers analysed bee behaviour and gave them memory tests, such as navigating paths using colour cues, in order to learn more about the structure of insect brains.

The project strengthened the argument that bee brains are more complex than previously thought. ‘Most findings are saying that insects are more than simple machines, which comes from studies in the honeybee,’ said Prof. Giurfa. ‘(But) the entrance region of the mushroom body shows a level of complexity and the studies show that this complexity is not rigid, it is plastic.’

This means its structure is changing all the time, which mirrors how human brains work. ‘(Bee) brains are capable of sophisticated performances such as learning concepts and rules; they are incredible organs and they need to be defended,’ said Prof. Giurfa

To further advance understanding of the mushroom bodies and how they function in different species, the project is being co-led by Professor James Traniello at Boston University in the US, an expert in ant evolutionary neurobiology.

Ants, which are related to honeybees, have brains that may be 100 times smaller, and due to their minute size, provide insights into how insect brains are structured.

‘What happens to neural tissue at an extremely small size?’ asked Prof. Traniello. ‘Are you losing neurons, are neurons becoming more efficient in their actions, how many neurons do you have to string together to form a circuit that enables behaviours as complex as what you would see in ants? How does the collective intelligence of an ant colony impact the structure of the brain?’

If BrainiAnt can answer these questions, it would provide a clearer picture of the evolution and function of ant brains.

‘The next step is trying to understand the genes that are involved in regulating brain size, compartment variability, metabolism and other functions,’ said Prof. Traniello.

He added that a better understanding of neural tissue could also help to guide attempts to genetically engineer bees so their brains are resistant to environmental threats like neonicotinoids. Although far off, it could mean that bees, and the benefits they bring to the environment, will have a more secure future.

The research in this article was funded by the EU.

*

The issue

One in ten pollinating insects is on the verge of extinction, and a third of bee and butterfly species are in decline.

On 1 June, the European Commission launched a proposal to tackle this problem at an EU level. It includes a new monitoring process to collect quality data and identify trends, action plans to protect insect habitats and incentives for businesses such as those in the agrifood sector, to contribute to conservation.

The proposal, known as the EU Pollinators Initiative, has a number of short-term actions to be taken before 2020, at which point the progress will be reviewed.

This post Decoding the honeybee dance could lead to healthier hives was originally published on Horizon: the EU Research & Innovation magazine | European Commission.